■ 基本信息
原核抗性: | Amp |
克隆菌株: | DH5a |
| 培養(yǎng)條件: | 37度 |
備注: |
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■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
Available separately or as part of the BacPAK? Baculovirus Expression System (Cat. No. 631402). pBacPAK9 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites which facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK9 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and an ampicillin resistance gene for selection in E. coli.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1874 pBacpAK9昆蟲胞內(nèi)質(zhì)粒.txt
