■ 基本信息
別名: | pFastBac Dual |
啟動子: | P10+PH |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 廣宿主系列，昆蟲細(xì)胞載體 |
質(zhì)粒大小: | 5238bp |
原核抗性: | Amp |
真核抗性: | Gen |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 昆蟲細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達(dá) |
5'測序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'測序引物: | pFastBac-R:ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 多框空載
|
原核抗性: | Amp,Gen |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pFastBac Dual昆蟲細(xì)胞表達(dá)載體包含有兩個啟動子，能夠同時表達(dá)兩個外源基因，一個表達(dá)基因是polyhedrin (PH)啟動子控制，另一個外源基因是由p10啟動子控制。
pFastBacDual 載體的特點(diǎn)是在單個載體上有兩個啟動子，用于在使用 Bac-to-Bac? 桿狀病毒表達(dá)系統(tǒng)(Bac-to-Bac? Baculovirus Expression System) 時在昆蟲細(xì)胞中同時表達(dá)兩個蛋白質(zhì)。該載體具有兩個強(qiáng)啟動子，多角體蛋白啟動子和p10 啟動子，因此可實現(xiàn)高水平表達(dá)。包含 CAT和β-葡萄糖醛酸酶 (gus) 的表達(dá)對照。
The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:
Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac? E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1045pFastBacDual昆蟲雙框質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
