■ 基本信息
啟動(dòng)子: | PH |
復(fù)制子: | pBR322 |
終止子: | polyhedrin 3 |
質(zhì)粒分類(lèi): | 昆蟲(chóng)細(xì)胞桿狀病毒組成型表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 9632bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 昆蟲(chóng)細(xì)胞 |
誘導(dǎo)方式: | 組成性表達(dá)，無(wú)需誘導(dǎo) |
5'測(cè)序引物: | Polyhedrin-F：AAATGATAACCATCTCGC |
3'測(cè)序引物: | Polyhedrin-R：GTCCAAGTTTCCCTG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.
? Multiple cloning site polylinker, positioned in opposite orientations
? Recombination sequences for insertion into the baculovirus genome
? Polyhedrin enhancer-promoter sequences for high expression of recombinant protein Features for growth and maintenance in E. coli:
? Ampicillin gene for selection
? pMB1 origin of replication from pUC19 for high copy number growth
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1043pVL1392昆蟲(chóng)胞內(nèi)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
