■ 基本信息
啟動子: | P43 |
質粒分類: | 廣宿主系列,枯草桿菌載體 |
質粒大小: | 3787bp |
原核抗性: | Kan |
克隆菌株: | BS168 |
培養(yǎng)條件: | 37度 |
表達宿主: | 枯草芽孢桿菌 |
| 5'測序引物: | 根據(jù)序列設計引物 |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 枯草桿菌 |
質粒用途: | 蛋白表達,分泌表達 |
片段類型: | ORF |
片段物種: | 空載體
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原核抗性: | Kan |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.
■ 質粒圖譜
■ 質粒序列
質粒序列可參考(具體以測序為準)下載:
ZK1024pWB980枯草分泌質粒.txt
質粒只保證關鍵序列正確,不保證表達效果。