■ 基本信息
啟動子: | CYC1 |
復(fù)制子: | pUC ori，F(xiàn)1 ori，2μ ori |
終止子: | CYC1 terminator |
質(zhì)粒分類: | 酵母細胞質(zhì)粒；酵母雜交質(zhì)粒；雙雜交類質(zhì)粒 |
質(zhì)粒大小: | 7599bp |
質(zhì)粒標(biāo)簽: | C-Cub，C-LexA,VP16 |
原核抗性: | Kan |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母菌 |
培養(yǎng)條件:
| 30℃，YPD，有氧
|
備注: | 高拷貝 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | LEU2
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pBT3-C是一個用于膜蛋白酵母雙雜交實驗的質(zhì)粒。The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find novel
interacting proteins (requires purchase of a separate cDNA library from Dualsystems)
■ 質(zhì)粒圖譜
