■ 基本信息
啟動(dòng)子: | CYC1 promoter |
復(fù)制子: | pUC ori，F(xiàn)1 ori，2μ ori |
終止子: | CYC1 terminator |
質(zhì)粒分類: | 酵母細(xì)胞質(zhì)粒；酵母雜交質(zhì)粒；雙雜交類質(zhì)粒 |
質(zhì)粒大小: | 6200bp |
質(zhì)粒標(biāo)簽: | N-NubG；N-HA |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 酵母菌 |
培養(yǎng)條件: | 30℃，YPD，有氧 |
5'測(cè)序引物: | CYC1-F：CTTTCCTTATACATTAGGACC |
3'測(cè)序引物:
| CYC1-R：GGGACCTAGACTTCAGGTTG |
備注: | 高拷貝質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | TRP1
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pPR3-N是一個(gè)用于膜蛋白酵母雙雜交實(shí)驗(yàn)的質(zhì)粒。The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find novel
interacting proteins (requires purchase of a separate cDNA library from Dualsystems)
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK987pPR3-N膜蛋白雙雜交質(zhì)粒.txt
