■ 基本信息
啟動(dòng)子: | 35s |
復(fù)制子: | pVS1V |
質(zhì)粒分類(lèi): | 植物系列，蛋白過(guò)表達(dá)載體 |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測(cè)序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: |
|
片段物種: |
|
原核抗性: | Kan |
真核抗性: | Hyg
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use these vectors to create fusions of gusA with their gene of interest. These vectors contain the pUC18 polylinker-lacZa.
