■ 基本信息
啟動(dòng)子: | hU6 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒大小: | 4893bp |
質(zhì)粒標(biāo)簽: | EGFP |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
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片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pGenesil-1 plasmid was obtained by the transformation of pEGFP-C1 plasmid. PEGFP-C1 plasmid has been widely used in mammalian cells for many years, it has fluorescent protein expression, it is very convenient to detect the effect of. When your target gene was not detected by conventional fluorescence detection, the pGenesil-1 plasmid encoding shRNAs was a better choice.
■ 質(zhì)粒圖譜
