■ 基本信息
啟動(dòng)子: | EF1α |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，抗體表達(dá)載體 |
質(zhì)粒大小: | 4194bp |
原核抗性: | Zeo |
真核抗性: | Zeo |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
備注: | 哺乳細(xì)胞抗體表達(dá)載體 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Zeo |
真核抗性: | Zeo
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pFUSE-Fc is a family of plasmid developed to facilitate the construction of Fc-fusion proteins by fusing the effector region of a protein to the Fc region of an immunoglobul in G (IgG).
pFUSE-Fc plasmids yield high levels of Fc-fusion proteins. The level of expression is usually in the μg/mL range. They can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems.
pFUSE-Fc2 (IL2ss) plasmids allow the secretion of Fc-Fusion proteins.They contain the IL2 signal sequence (IL2ss) for the generation of Fc- Fusion proteins derived from proteins that are not naturally secreted. As Fc- Fusion proteins are secreted, they can be easily detected in the supernatant of pFUSE-Fc-transfected cells by SDS-PAGE. Furthermore, functional domains can be identified by immunoblotting and ligand blotting.Fc-Fusion proteins can be easily purified by single-step protein A or protein G affinity chromatography.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK703pFUSE-hIgG1e1-Fc2哺乳抗體質(zhì)粒.txt
