■ 基本信息
別名: | 有背景;Plasmid #22010 |
啟動子: | CMV |
復制子: | pUC |
終止子: | hGH poly(A) signal |
質粒分類: | 哺乳細胞，雙分子熒光互補載體 |
質粒大小: | 5216bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
BiFC-VN173 by pFlag-CMV Xba I and BamHI carrier in the site of insertion of the VN173 gene, the plasmid is high copy plasmid, VN173 gene derived from Vitoria victoria. Visualization of Aequorea Aequorea ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008).
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK517pBiFC-VN173雙分熒光質粒.txt
