■ 基本信息
啟動(dòng)子: | SV40 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
質(zhì)粒大小: | 5659bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號(hào)報(bào)告 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: | Fluc
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■ 質(zhì)粒簡(jiǎn)介
Pp53-TA-luc can be transfected into cells by conventional cell transfection method. The biyuntian firefly firefly firefly firefly luciferase reporter gene assay kit detection (RG005/RG006) or dual luciferase reporter gene assay kit (RG027/RG028).
Ultraviolet irradiation, ion irradiation can lead to the role of DNA damage or reagent can activate p53, can be used as a positive control pp53-TA-luc report gene detection.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK493pP53-TA-luc哺乳報(bào)告質(zhì)粒.txt
