■ 基本信息
啟動子: | SV40 |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細胞，信號通路報告載體 |
質(zhì)粒大小: | 6084bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃ |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| RVP3:CTAGCAAAATAGGCTGTCCC
|
3'測序引物: | 根據(jù)序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 信號報告 |
片段類型: | Promoter
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Hyg
|
熒光標記: | Fluc
|
■ 質(zhì)粒簡介
The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK479pGL4.29[luc2P-CRE-Hygro]哺乳報告質(zhì)粒.txt
